Table 1.
Primer sets for qRT-PCR analysis
| Gene | Direction | Sequence (5′–3′) | Product size (bp) |
|---|---|---|---|
| NANOG | Forward | TTTGTGGGCCTGAAGAAAACT | 91 |
| Reverse | AGGGCTGTCCTGAATAAGCAG | ||
| SOX2 | Forward | GCCGAGTGGAAACTTTTGTCG | 145 |
| Reverse | GGCAGCGTGTACTTATCCTTCT | ||
| OCT4 | Forward | CTGGGTTGATCCTCGGACCT | 121 |
| Reverse | CCATCGGAGTTGCTCTCCA | ||
| BRACHYURY | Forward | CAGTGGCAGTCTCAGGTTAAGAAGGA | 122 |
| Reverse | CGCTACTGCAGGTGTGAGCAA | ||
| MESP1 | Forward | AGCCCAAGTGACAAGGGACAACT | 82 |
| Reverse | AAGGAACCACTTCGAAGGTGCTGA | ||
| NKX2-5 | Forward | CAAGTGTGCGTCTGCCTTT | 190 |
| Reverse | CAGCTCTTTCTTTTCGGCTCTA | ||
| TNNT2 | Forward | TTCACCAAAGATCTGCTCCTCGCT | 111 |
| Reverse | TTATTACTGGTGTGGAGTGGGTGTGG | ||
| SHOX2 | Forward | CAAAGAGGATGCGAAAGGGAT | 122 |
| Reverse | AGTGGGTCTCGTCAAAAAGCC | ||
| TBX18 | Forward | GACGATCTTTCTCCCATCAAGC | 124 |
| Reverse | CTATCTTCAGGCGAGTAATCTGC | ||
| TBX3 | Forward | CCCGGTTCCACATTGTAAGAG | 104 |
| Reverse | GTATGCAGTCACAGCGATGAAT | ||
| HCN4 | Forward | TGGACACCGCTATCAAAGTGG | 157 |
| Reverse | CTGCCGAACATCCTTAGGGA | ||
| MYL7 | Forward | AAGCCATCCTGAGTGCCTTC | 127 |
| Reverse | AACATCTGCTCCACCTCAGC | ||
| MYL2 | Forward | TGAGAGACACCTTTGCTGCC | 139 |
| Reverse | GGGTCCGCTCCCTTAAGTTT | ||
| MSX2 | Forward | CTGGTGAAGCCCTTCGAGAC | 133 |
| Reverse | ATATGTCCTCCTACTCCTGCCC | ||
| TBX2 | Forward | TACGCTTGTACGAGGAGCAC | 157 |
| Reverse | CACGACTTCTCCTCAGCTCG | ||
| ISL1 | Forward | TCCCTATGTGTTGGTTGCGG | 194 |
| Reverse | CATTTGATCCCGTACAACCTGA | ||
| Cx30.2 | Forward | CCGAGAACCTTGCCTTGGTA | 98 |
| Reverse | GACAGAAACCGCTGACTCCA | ||
| Cx43 | Forward | TACCAAACAGCAGCGGAGTT | 139 |
| Reverse | TGGGCACCACTCTTTTGCTT | ||
| Cx40 | Forward | AGAGTGTGAAGAAGCCCACG | 70 |
| Reverse | AACAGATGCCAAAACTTCTGCT | ||
| HCN1 | Forward | GCCATGCTGAGCAAGTTGAG | 178 |
| Reverse | TCAGCAGGCAAATCTCTCCA | ||
| KCND2 | Forward | GGGTCTTCGGCTAGCAAGTT | 91 |
| Reverse | GCACCATGTCACCATACCCTA | ||
| KCNK2 | Forward | TGGAACAAGACTCCTTGCTGG | 150 |
| Reverse | CTGCCGAACATCCTTAGGGA | ||
| KCNN4 | Forward | CCGAGAGGCAGGCTGTTAAT | 81 |
| Reverse | AGCCGATGGTCAGGAATGTG | ||
| KCNJ5 | Forward | CCCACAACAGGGAGAGGTTC | 170 |
| Reverse | AGCCATAGCTGGGATGTTGTT | ||
| KCNQ1 | Forward | GGGCCGCGTCTACAACTTC | 169 |
| Reverse | CAGCACGATCTCCATCCAGAA | ||
| CACNA1A | Forward | GTCTGGGGAAGAAGTGTCCG | 151 |
| Reverse | GCTCCTCCCTTGGCAATCTT | ||
| CACNB1 | Forward | CCAGTGCCAAACAGAAGCAG | 181 |
| Reverse | CGAGTGATGGAGATCCTGCC | ||
| CACNA1C | Forward | GACGTGCTGTACTGGGTCAA | 125 |
| Reverse | AACTCTCCGCTAAGCACACC | ||
| SCN5A | Forward | GGAGGAGTCCAGCAAGCAG | 194 |
| Reverse | AACTGTCCTCTGGGGTCTCA | ||
| SCN3B | Forward | ACGCATTCTGTAGCCCAGAC | 156 |
| Reverse | CTTCCAAGGCTCTCGCCTC |