Table 4.
Primers used to confirm the presence of E. coli O157:H7 and virulence genes
| Target gene | Primer name | Primer sequence (5′–3′) | Amplification size (bp) |
|---|---|---|---|
| rfbE | O157AF | AAGATTGCGCTGAAGCCTTTG | 497 |
| O157AR | CATTGGCATCGTGTGGACAG | ||
| fliC | FLICH7 F | GCGCTGTCGAGTTCTATCGAGC | 625 |
| FLICH7 R | CAACGGTGACTTTATCGCCATTCC | ||
| uidA | PT-2 | GCGAAAACTGTGGAATTGGG | 252 |
| PT-3 | TGATGCTCCATAACTTCCTG | ||
| hlyA | hlyA F | GCATCATCAAGCGTACGTTCC | 534 |
| hlyA R | AATGAGCCAAGCTGGTTAAGCT | ||
| stx2 | Stx2F | GGCACTGTCTGAAACTGCTCC | 255 |
| Stx2R | TCGCCAGTTATCTGACATTCTG | ||
| stx1 | Stx1F | ATAAATCGCCATTCGTTGACTAC | 180 |
| Stx1R | AGAACGCCCACTGAGATCATC |