Table 1. Yield and Purity of HBx Fusion Proteinsa.
yield (mg/L) | % HBx fusion | % GroEL | |
---|---|---|---|
GST-HBx | 0.4 ± 0.3 | 3.5 ± 2.2 | 8.4 ± 2.2 |
MBP-HBx | 8.9 ± 1.3 | 74.0 ± 0.4 | 11.7 ± 0.04 |
DsbC-HBx | 6.6 ± 0.6 | 93.8 ± 0.5 | 0.1 ± 0.01 |
NusA-HBx | 28.6 ± 4.7 | 82.6 ± 0.5 | 0.2 ± 0.01 |
The percentages of the HBx fusions and GroEL in the samples were quantified by mass spectrometry using the emPAI values.