Table 2. Factors influencing the performance of Coronavirus disease (COVID-19) outbreak preparedness EQA participants, logistic regressions on combined responses reported for all samples in the panel, April/May 2020 (n = 4,168).
| Possible technical influence factors (grouped by methods) | Total responses per group | Percentage correct results | p value | Odds ratio | 95% confidence interval | |
|---|---|---|---|---|---|---|
| % | Responses (n) | |||||
| Extraction method (overall) | 4,168 | 96.3 | 4,012 | < 0.001* | ||
| Any other method (incl. seven in-house) | 1,152 | 94.6 | 1,090 | Reference level | ||
| Abbott m2000sp | 168 | 99.4 | 167 | 0.026* | 9.50 | 1.31–68.97 |
| BD MAX ExK TNA-3 | 144 | 90.3 | 130 | 0.040* | 0.53 | 0.29–0.97 |
| Roche Cobas Omni | 304 | 99.3 | 302 | 0.003* | 8.59 | 2.09–35.32 |
| PSS MagDEA Dx | 72 | 100 | 72 | 0.974 | NA | NA |
| Roche MagNA Pure 96 DNA and Viral | 336 | 96.7 | 325 | 0.119 | 1.68 | 0.88–3.23 |
| Promega Maxwell RSC (Viral/Blood) | 176 | 95.5 | 168 | 0.644 | 1.19 | 0.56–2.54 |
| BioMérieux NucliSENS easyMAG | 544 | 98.4 | 535 | < 0.001* | 3.38 | 1.67–6.86 |
| Qiagen QIAamp Viral RNA Mini Kit | 184 | 98.9 | 182 | 0.023* | 5.18 | 1.26–21.35 |
| Qiagen QIAsymphony DSP Virus | 144 | 97.2 | 140 | 0.189 | 1.99 | 0.71–5.56 |
| Seegene STARMag Universal Cart. | 360 | 98.1 | 353 | 0.009* | 2.87 | 1.30–6.32 |
| Cepheid Xpert Xpress SARS-CoV-2 | 376 | 94.4 | 355 | 0.880 | 0.96 | 0.58–1.60 |
| Not specified | 208 | 92.8 | 193 | 0.295 | 0.73 | 0.41–1.31 |
| Amplification method (overall) | 4,168 | 96.3 | 4,012 | < 0.001* | ||
| Any real-time in-house PCR | 952 | 96.7 | 921 | Reference level | ||
| Abbott RealTime SARS-CoV-2 | 136 | 99.3 | 135 | 0.138 | 4.54 | 0.62–33.53 |
| Altona RealStar SARS-CoV-2 RT-PCR | 224 | 99.1 | 222 | 0.072 | 3.74 | 0.89–15.73 |
| Cepheid Xpert Xpress SARS-CoV-2 | 408 | 94.9 | 387 | 0.098 | 0.62 | 0.35–1.09 |
| Certest VIASURE SARS-CoV-2 | 136 | 91.9 | 125 | 0.008* | 0.38 | 0.19–0.78 |
| Certest VIASURE SARS-CoV-2 S gene | 152 | 90.8 | 138 | < 0.001* | 0.33 | 0.17–0.64 |
| Elitech GeneFinder COVID-19 Plus | 200 | 95.0 | 190 | 0.230 | 0.64 | 0.31–1.33 |
| Roche cobas SARSCoV2 | 296 | 99.3 | 294 | 0.029* | 4.94 | 1.18–20.77 |
| Seegene Allplex 2019-nCoV | 552 | 98.9 | 546 | 0.013* | 3.06 | 1.27–7.39 |
| TIB MOLBIOL LightMix Modular | 216 | 98.6 | 213 | 0.153 | 2.39 | 0.72–7.89 |
| Any other commercial test kit | 896 | 93.9 | 841 | 0.004* | 0.52 | 0.33–0.81 |
NA: not applicable.
* Statistically significant; p ≤ 0.05 (for details see text).