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. 2020 Jul 14;48(7):0300060520930853. doi: 10.1177/0300060520930853

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Extraction, culture, and identification of PBMCs from donors of different ages. (A): (a) PBMCs isolated from younger donors, (b) PHA-activated PBMCs from younger donors (scale bar: 200 μm). (B): (a) PBMCs isolated from older donors, (b) PHA-activated PBMCs from older donors (scale bar: 200 μm). (C): Analysis of CD3, CD28, and CD95 surface markers in PBMCs from younger donors. (D): Analysis of CD3, CD28, and CD95 surface markers in PBMCs from older donors. (E): Rate of CD28 expression decreased with age and rate of CD95 expression increased with age. Data represent three independent experiments. Values are expressed as mean ± standard deviation (**p < 0.01, ****p < 0.0001, n.s., no significance).

Abbreviations: CD, cluster of differentiation; hPDLSCs, human periodontal ligament stem cells; OPBMCs, older peripheral blood mononuclear cells; PBMCs, peripheral blood mononuclear cells; PHA, phytohemagglutinin; YPBMCs, younger peripheral blood mononuclear cells.