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. 2020 Jul 14;48(7):0300060520930853. doi: 10.1177/0300060520930853

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — T lymphocyte proliferation from donors of different ages. (A): Effects of hPDLSC addition on PHA-stimulated PBMC proliferation under different experimental conditions. (B): Effect of hPDLSCs on two-way mixed lymphocyte reaction. Number of EdU⁺ cells per 10,000 viable lymphocytes after incubating cells. Data represent three independent experiments. Values are expressed as mean ± standard deviation (#, p < 0.05, compared with PHA-stimulated PBMCs in same age group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Abbreviations: EdU, 5-ethynyl-2′-deoxyuridine; de-hPDLSCs, delayed addition of human periodontal ligament stem cells; hPDLSCs, human periodontal ligament stem cells; MLR, mixed lymphocyte reaction; OPBMCs, older peripheral blood mononuclear cells; PBMCs, peripheral blood mononuclear cells; PHA, phytohemagglutinin; re-PHA(hPDLSCs), peripheral blood mononuclear cells reactivated by phytohemagglutinin after inhibition by human periodontal ligament stem cells); transwell(hPDLSCs), transwell culture of peripheral blood mononuclear cells and human periodontal ligament stem cells; YPBMCs, younger peripheral blood mononuclear cells.