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. 2020 Jul 14;48(7):0300060520930853. doi: 10.1177/0300060520930853

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Quantitative polymerase chain reaction analysis of (A) IL-2, (B) IFN-γ, and (C) TNF-β gene expression levels in PBMCs from younger and older donors. Enzyme-linked immunosorbent assays of (D) IL-2, (E) IFN-γ, and (F) TNF-β protein levels in supernatant of PBMCs from younger and older donors. Data represent three independent experiments. Values are expressed as mean ± standard deviation (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Abbreviations: hPDLSCs, human periodontal ligament stem cells; IFN, interferon; IL, interleukin; OPBMCs, older peripheral blood mononuclear cells; PBMCs, peripheral blood mononuclear cells; PHA, phytohemagglutinin; TNF, tumor necrosis factor; YPBMCs, younger peripheral blood mononuclear cells.