Figure 3.

Mutations made in HP1α can be translated to the CBX1 and CBX3 chromodomains. a) Sequence alignment of wild-type Drosophila melanogaster HP1α with human homologs CBX 1,3, and 5. Green regions of the β-sheet (denoted as arrows) indicate binding interface with the histone tail. Beneficial mutations identified in HP1α along with their corresponding locations in CBX homologs are highlighted. Note that CBX1 and CBX3 are double mutants while CBX5 is a triple mutant. b) and c) ITC binding curves for the wild-type and mutant CBX1 chromodomains. d) Binding results for ITC experiments with the CBX1 and CBX3 wild-type and mutant chromodomains. Data shown are representative of ≥ 2 ITC binding experiments.