Figure 6.

Engineered chromodomains bind to H3K9 methylated nucleosomes more effectively than wild-type or a H3K9me3 antibody. a) Nucleosome binding of the CBX1 constructs used previously as compared to a commercial anti-H3K9me3 antibody at 10 and 100 nM (upper) and Ponceau stain to show equivalent loading (lower). All images are representative of n ≥ 3 experiments. b) Summary of findings for the study, describing the increase in binding derived from mutagenesis and/or tandem arrangement of chromodomains.