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. Author manuscript; available in PMC: 2020 Jul 16.
Published in final edited form as: J Immunol. 2011 Mar 2;186(7):4244–4252. doi: 10.4049/jimmunol.1003318

Figure 7. ADAM10 overexpression and γ-secretase inhibition disrupt Notch signaling and direct myeloid cell development.

Figure 7.

Flow cytometric analysis of LSK differentiation after 29 days of LSK co-culture with (A) OP9-DL1 or (B) OP9-GFP stromal cells as described in the Methods. Compound E, GI254023X, or control DMSO was added to media to inhibit γ-secretase or ADAM10 activity, respectively. DMSO-treated plots are representative of 4 independent experiments; plots of inhibitor-treated cultures are representative of 2 independent experiments. Numbers on plots indicate the percentage of gated live cells based upon forward and side scatter.