Figure 7.

Validation of the role of Rho-GTPases in nonatopic T-helper–cell responses. siRNA-based silencing of CDC42 (cell division cycle 42; black bars), as compared with mock control (gray bars), was associated with downregulation of IFNγ, but not of IL-4, expression. Although it did not reach statistical significance, a 50% downregulation of TNF (tumor necrosis factor) expression was observed in cells with CDC42 silencing. TRAF3 (TNF receptor–associated factor 3) and HHEX (hematopoietically expressed homeobox protein), included as additional controls, supported that the findings were not due to off-target effects of gene silencing.