Table 1.
Expression of Host Proteins Potentially Relevant to SARS-CoV-2 Infection in Undifferentiated and Differentiated Airway Basal Cell Lines*
| Gene | BCi-NS1.1† |
hSABCi-NS1.1‡ |
||
|---|---|---|---|---|
| Basal Cell Baseline | Air–Liquid Interface Day 28 | Basal Cell Baseline | Air–Liquid Interface Day 28 | |
| ACE2 | 0.4 | 1.7 | 0.4 | 2.8 |
| ADAM10 | 23.8 | 13.6 | 23.4 | 21.8 |
| ADAM17 | 9.6 | 6.9 | 14.0 | 11.8 |
| TMPRSS2 | 3.3 | 25.4 | 12.2 | 15.2 |
| TMPRSS11A | 0 | 0.5 | 0.5 | 0 |
| TMPRSS11D | 0.1 | 0.7 | 0.4 | 0.2 |
| FURIN | 14.9 | 12.5 | 46.8 | 29.5 |
| CTSL | 37.5 | 33.1 | 74.7 | 67.0 |
| PI4KB | 18.0 | 28.6 | 19.2 | 22.6 |
Definition of abbreviations: FPKM = fragments per kilobase of exon per million fragments sequenced; SARS-CoV-2 = severe acute respiratory syndrome coronavirus 2.
*
Expression assessed by RNA sequencing (HiSeq 4000; Illumina); data is presented in FPKM.
†
The BCi-NS1.1 line was derived from large airway epithelium basal cells of a healthy nonsmoker and differentiated on air–liquid interface for 28 days (20).
‡
The hSABCi-NS1.1 line was derived from small airway epithelium basal cells of a healthy nonsmoker and differentiated on air–liquid interface for 28 days (21).