Fig 4. Impact of the length of exposure of arrested mature schizonts to ML10 or Compound 2 on parasite viability.

(A) Cultures containing mature schizonts were maintained for 0–12 hours in RPMI-1640 supplemented with AlbuMax containing either DMSO, 25 nM ML10 or 1.5 μM Compound 2 and subsequently washed and allowed to egress and invade fresh red blood cells. The resulting parasitemia was determined by flow cytometry and reflects the survival of the parasites in the compound. Shown is a representative experiment of five independent experiments; each experiment consisted of three technical replicates. The statistical significance of the difference between the parasitemia of the culture treated for one hour and the subsequent samples was determined using an unpaired t-test; **p≤0.01, ***p≤0.001, whereas no symbol indicates no statistical significance. (B) Giemsa-stained parasite smears were produced immediately prior to removal of the compound to ascertain the effect of incubation in the presence of the compound on the morphology of the parasites. Numbers above the images denote the length of time (in hours) the parasites had been exposed to the compound.