Fig 1. Cell sorting of CD24^- and CD24⁺ subpopulations of MCF7 cells.

(A) Histograms of ESA-FITC positive cells that were later sorted for CD44-APC positive and CD24-PE negative (1.6%). (B) Microphotograph under light microscope of mammospheres from CD24^- cells in culture. Representative images of spheres stained with hematoxylin/eosin, E-cadherin or P-cadherin. Scales bars are included. (C) Immunoblot of total c-Src, SrcDN, p-Y397-Fak/Fak, and E-cadherin, using α-Tubulin expression as loading control from CD24^- cells forming mammospheres and CD24⁺ cells in absence (Control) or presence of Doxy (2 μg/mL) for induction of SrcDN during three passages. The ratios of the proteins Total c-Src, SrcDN, and E-cadherin with the loading control α-Tubulin, or pY397-Fak/Fak were calculated and referred to -Doxy (Control) considered as 1. These are representative results from 3 independent experiments.