Fig 4. Expression of SrcDN reduced glycolysis in MCF7 mammosphere-derived cells.
Control and Doxy-treated (2 μg/mL) mammospheres from the 3rd generation were used to study glycolysis. (A) Determination of glucose consumption. (B) Lactate production, intracellular lactate. (C) Enzymatic activity of hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH). (***p<0.001). (D) Expression of Glut-1, total c-Src, HK2, pY10-LDHA, LDHA, and MCT-1, was determined by immunoblotting, employing ß-Actin as a loading control. These are representative results from 3 independent experiments. The ratios refer to -Doxy considered as 1.