FIGURE 3.

Effects of sitravatinib on IC₅₀ values of antineoplastic drugs in ABCB1‐ or ABCC10‐transfected HEK293 cell line and its parental cell line HEK293 cell line transfected with pcDNA3.1. A. IC₅₀ values of paclitaxel, doxorubicin, vincristine, and cisplatin incubated with or without inhibitor for 72 h on HEK293 cell line transfected with erither pcDNA3.1 vector or ABCB1 expression plasmid. Verapamil at 3 µmol/L acts as positive control. B. Cytotoxicity of paclitaxel and cisplatin in the absence or presence of inhibitor for 72 h on HEK293/pcDNA3.1 and ABCC10‐transfected HEK293 cell line. Cepharanthine at 3 µmol/L functions as positive control. Data are showed as mean ± SD, representive of 3 independent experiments. *, P < 0.05 indicates statistically significant. Abbreviations: ABCB1, ATP‐binding cassette subfamily B member 1; ABCC10, ATP‐binding cassette subfamily C member 10; SD, standard deviation