Fig 4. Effects of nocodazole treatment on the length of major neurites and primary neurite number.

Mouse neurons in culture were treated with 50nM nocodazole 4h after plating. After 2DIV the neurons on coverslips were fixed and stained for tubulin and Hoescht before imaging and AutoNeuriteJ quantifications. (A) Nocodazole treatment reduce the size of the longest neurite by 25% (n = 3210 for control, n = 2619 for nocodazole treated) but not on axons of polarized cells (n = 297 for control and n = 175 for treated). (B) Nocodazole treatment reduces the number of primary neurites of polarized (3.70 ±0.14 vs 2.72 ±0.12 for treated) or unpolarized (4.43 ±0.14 vs 3.51 ±0.13 for treated) neurons. p values were obtained from Mann-Whitney tests.