Table 2. Effect of narrow-band UVA light on bacteria based on time exposure using varying intensities at 1 cm from the target.
Microorganism | UVA Intensity (μW/cm2) | Group | Baseline | 20 min | % Reduction | Log Reduction | P value | 40 min | % Reduction | Log Reduction | P value |
---|---|---|---|---|---|---|---|---|---|---|---|
CFUx107/mL | CFUx107/mL | CFUx107/mL | |||||||||
Escherichia coli GFP | 500 | Exposed | 0.25 | 0.16 | 32.87 | 0.17 | 0.26 | 0.13 | 53.80 | 0.34 | 0.06 |
Control | 0.25 | 0.24 | 0.28 | ||||||||
1000 | Exposed | 0.14 | 0.12 | 20.97 | 0.10 | 0.38 | 0.08 | 55.50 | 0.35 | 0.01 | |
Control | 0.14 | 0.15 | 0.18 | ||||||||
2000 | Exposed | 0.26 | 0.14 | 41.96 | 0.24 | 0.17 | 0.04 | 86.77 | 0.88 | 0.01 | |
Control | 0.26 | 0.23 | 0.30 | ||||||||
3000 | Exposed | 0.19 | 0.02 | 89.09 | 0.96 | <0.001 | No growth | * | * | 0.01 | |
Control | 0.19 | 0.15 | 0.19 | ||||||||
Pseudomonas aeruginosa | 500 | Exposed | 0.49 | 0.27 | 24.79 | 0.12 | 0.17 | 0.24 | -23.87 | -0.09 | 0.47 |
Control | 0.49 | 0.36 | 0.19 | ||||||||
1000 | Exposed | 0.49 | 0.24 | 25.24 | 0.13 | 0.01 | 0.10 | 37.07 | 0.20 | <0.001 | |
Control | 0.49 | 0.40 | 0.32 | ||||||||
2000 | Exposed | 0.28 | 0.06 | 69.07 | 0.51 | 0.17 | 0.03 | 78.37 | 0.67 | 0.05 | |
Control | 0.28 | 0.20 | 0.14 | ||||||||
3000 | Exposed | 0.40 | 0.07 | 77.42 | 0.65 | <0.001 | 0.02 | 89.23 | 0.97 | 0.01 | |
Control | 0.40 | 0.32 | 0.22 |
* No growth observed. Bactericidal threshold of >99.9% or 3 log reduction is met.