Fig 6. Proposed scheme for mixed NM2-minifilament assembly.

A: Different homo- and hetero-staggers are tested by trial-and-error and with rolling motion. B: The stable antiparallel dimer with overlap of 43–45 nm serves as main scaffold for minifilament nucleation. C: Newly added parallel NM2 are splayed away from the backbone. D: The mature minifilament has five crowns of three monomers each on both sides totalling 30 monomers.