Fig 2. EVs released by drug-resistant L. infantum parasites show altered particle profiles and EV secretion capacity.

(A) L. infantum WT and drug-resistant strains were compared in terms of number of particles per category (size) between 0 nm and 500 nm using NTA. One representative image of three experiments is shown (experiments 2 and 3 are shown in S1). (B) Total number of particles, from 0 nm to 500 nm, secreted by WT, Sb2000.1, MF200.5 and AmB1000.1. (C) Percentage of particles corresponding to 0–100 nm. (D) Percentage of particles corresponding to 100–200 nm. (E) Percentage of particles corresponding to 200–500 nm. In A–E, the results are representative of at least three independent experiments with similar data. Differences were statistically evaluated by unpaired t-test (*p < 0.05).