Table 3. Enriched proteins identified in EVs isolated from the Sb2000.1 L. infantum strain.
| Gene ID | Protein | Total Spectrum Count1 | (Trans)membrane protein | Unconventional secretion prediction2 | Signal peptide3 | |
|---|---|---|---|---|---|---|
| 1 | LINJ_07_0960 | Hypothetical protein conserved | 17.3 | yes | 0.693, but prediction of a peptide signal | yes |
| 2 | LINJ_30_3650 | 40S ribosomal protein S14 | 15.3 | no | yes (0.693) | no |
| 3 | LINJ_28_1050 | 40S ribosomal protein S14 | 15.3 | no | yes (0.695) | no |
| 4 | LINJ_13_1510 | SPRYdomain/HECT-domain (Ubiquitin-transferase) putative | 14.3 | no | sequence too long to be analyzed | |
| 5 | LinJ_15_0540 | Ecotin-like protein 3 | 10.0 | no | no (0.343) | no |
| 6 | LINJ_33_2070 | Ribosomal protein L37 | 6.7 | no | yes (0.783) | no |
| 7 | LINJ_34_3420 | Uncharacterized protein | 5.7 | yes | 0.825, but prediction of a peptide signal | yes |
| 8 | LINJ_16_0510 | Hypothetical protein conserved | 5.3 | yes | no (0.126) | yes |
| 9 | LINJ_02_0340 | Putative proteasome regulatory non-ATPase subunit 6 | 4.7 | no | no (0.097) | no |
| 10 | LINJ_27_0500 | Putative calpain-like cysteine peptidase | 4.0 | no | sequence too long to be analyzed |
|
| 11 | LINJ_19_0560 | Hypothetical protein conserved | 3.7 | yes | 0.653, but prediction of a peptide signal | yes |
| 12 | LINJ_22_0440 | Putative proteasome regulatory ATPase subunit 1 | 3.7 | no | yes (0.659) | no |
| 13 | LINJ_28_0090 | Adenylate cyclase-like protein | 3.3 | no | no (0.358) | no |
| 14 | LINJ_20_1310 | Domain of unknown function (DUF1935)—putative | 3.3 | no | no (0.599) | no |
| 15 | LINJ_21_0830 | Hypothetical protein conserved | 3.0 | no | no (0.402) | no |
| 16 | LINJ_28_3090 | Malate dehydrogenase | 3.0 | no | no (0.447) | no |
| 17 | LINJ_16_1550 | Putative kinesin | 2.7 | no | no (0.394) | no |
| 18 | LINJ_32_4040 | Cysteine peptidase—putative | 2.3 | no | yes (0.620) | no |
| 19 | LINJ_30_1710 | SmallGTP-binding protein Rab28 putative | 2.3 | no | no (0.450) | no |
| 20 | LINJ_01_0010 | Protein of unknown function (DUF2946) | 2.0 | yes | no (0.085) | yes |
| 21 | LINJ_29_2550 | Phosphodiesterase putative | 1.7 | no | no (0.354) | no |
| 22 | LINJ_25_1640 | Putative casein kinase I | 1.3 | no | no (0.559) | no |
| 23 | LINJ_31_1130 | Putative N-acyl-L-amino acid amidohydrolase | 1.3 | no | no (0.530) | no |
| 24 | LINJ_32_2610 | Hypothetical protein conserved | 1.3 | no | no (0.408) | no |
| 25 | LINJ_20_0160 | Hypothetical protein conserved | 1.0 | no | no (0.080) | no |
| Total number of proteins | 5 (20.0%) | 5 (20.0%) | 5 (20.0%) | |||
1 Mean Total Spectrum Count obtained with the LC–MS/MS analyses.
2 NM score calculated using the SecretomeP 2.0 Server. Proteins with an NM score higher than 0.600 that are not predicted to have a signal peptide are considered non-classically secreted proteins.
3Signal peptides were predicted using SignalP Server integrated in SecretomeP 2.0 Sever.