(A-E) ZIKV RNA loads in embryonic brains (n = 3 in mock, n = 8 in E6.5–8.5, n = 11 in E9.5–10.5, n = 9 in E15.5, 3–5 dams per group) (A), placenta (n = 3 in mock, n = 10 in E6.5–8.5, n = 8 in E9.5–10.5, n = 9 in E15.5, 3–4 dams per group) (B), amniotic fluid (n = 5 in mock, n = 10 in E6.5–8.5, n = 9 in E9.5–10.5, n = 8 in E15.5, 3–5 dams per group) (C), dam cortex (n = 3 in mock, n = 8 in E6.5–8.5, n = 7 in E9.5–10.5, n = 4 in E15.5) (D) and dam serum (n = 3 in mock, n = 8 in E6.5–8.5, n = 8 in E9.5–10.5, n = 4 in E15.5) (E), measured by RT-qPCR. The dashed line represents the limits of detection (LOD). Non-parametric Kruskal Wallis test with Dunn’s multiple comparisons. * P <0.05, ** P <0.01, *** P <0.001 and **** P <0.0001.