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Caco-2 cells were incubated with 2mg/mL A. simplex CE. Basolateral and apical compartments were analysed for the presence of Ani s 4 at indicated time points. Results are expressed as percentage of total concentration (apical + basolateral compartments) of Ani s 4. Lines denote results from independent experiments. Western blot of a representative case is shown (experiment ▲).
