Figure 5.

Leg compression and position are differentially coded by DRG sensory neurons. Unsupervised clustering of neuronal responses to the knee compression. Markov Cluster Analysis (MCA) revealed 4 major clusters of responders to knee compression depicted as “Ramp,” “Low,” “Mid,” and “High.” The “Ramp” cluster could be fitted by linear regression (A). MCA revealed 3 major clusters of cells depicted as “Const,” “Flexion,” or “Extension” following leg movement. Because of the lack of differences in the clusters between the sham and cancer animals, groups were pooled (Fig. 6) (B). Example normalised GCaMP6s fluorescence traces from DRG neurons of the identified knee compression clusters. Pink line indicates the surge in cuff pressure (C). Example normalised GCaMP6s fluorescence traces from DRG neurons of the identified movement-evoked clusters. Pink line indicates events: higher points are reflecting extension (Ex) and lower flexion (Fl), the leg along the body axis (D). DRG, dorsal root ganglia.