Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 13;161(8):1894–1905. doi: 10.1097/j.pain.0000000000001880

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.

This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 7. — Intratibial and peritibial afferent function in health and bone cancer. Fast Blue (FB)-traced neurons from the tibial cavity. Representative z-stack collected at the end of the in vivo imaging experiment to identify traced cells (blue) and GCaMP6s (green). Scale bars: 100 µm. Arrows indicate FB-traced neurons (A). Percentage of responders of all FB-traced tibial afferents within L3 and L4 DRG during knee compression in sham and CIBP rats. Data represent the mean ± SEM of n = 81 cells from 6 animals (sham) and 220 cells from 9 animals (cancer). Repeated-measures ANOVA: NS, nonsignificant (B). Fluorescence intensities of all responding FB+ neuronal cell bodies to knee compression. Pressure was increased in 50-mm Hg increments every 10 seconds. Data represent the mean ± SEM of n = 15 cells (sham) from 6 animals, and n = 34 cells from 9 animals (CIBP). Repeated-measures ANOVA: NS, nonsignificant (C). AAVrg/tdTomato (TD)-traced neurons innervating the muscle and periosteum (MP) surrounding tibia. Representative z-stack collected at the end of the in vivo imaging experiment to identify traced cells (red) and GCaMP6s (green). Scale bars: 100 µm. Arrows indicate TD-traced neurons (D). Percentage of responders of all TD-traced MP afferents within L3 and L4 DRG during knee compression in sham and cancer animals. Pressure was increased in 50-mm Hg steps every 10 seconds (see methods for more). Data represent the mean ± SEM of n = 14 cells from 3 animals (sham) and 43 cells from 5 animals (cancer). Repeated-measures ANOVA: NS, nonsignificant (E). Fluorescence intensities of all responding TD+ neuronal cell bodies to knee compression. Pressure was increased in 50-mm Hg increments every 10 seconds. Data represent the mean ± SEM of n = 9 cells (cancer) from 3 animals. No between-group comparison was performed because in sham, no cells responded (F). ANOVA, analysis of variance; CIBP, cancer-induced bone pain; DRG, dorsal root ganglia.