KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb | Cell signaling | Cat#5483 |
| TBK1/NAK (D1B4) Rabbit mAb | Cell signaling | Cat#3504 |
| Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb | Cell signaling | Cat #4947 |
| Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb | Cell signaling | Cat#3033 |
| NF-κB p65 (D14E12) XP® Rabbit mAb | Cell signaling | Cat#8242 |
| STING (D1V5L) Rabbit mAb (Rodent Preferred) | Cell signaling | Cat#50494 |
| GAPDH (D16H11) XP® Rabbit mAb | Cell signaling | Cat#5174 |
| LC3B Antibody | Novus | Cat#NB100–2220 |
| PE anti-mouse CD3 Antibody | Biolegend | Cat#100206 |
| FITC anti-mouse CD8a Antibody | Biolegend | Cat#100706 |
| APC anti-mouse CD45 Antibody | Biolegend | Cat#103112 |
| PE/Dazzle™ 594 anti-mouse IFN-γ Antibody | Biolegend | Cat#505845 |
| FITC anti-mouse TNF-α Antibody | Biolegend | Cat#506304 |
| PE anti-mouse Perforin Antibody | Biolegend | Cat#154305 |
| APC-Granzyme B | Invitrogen | Cat#17-8898-80 |
| CD3e Monoclonal Antibody (145–2C11) | Invitrogen | Cat#16-0031-81 |
| CD28 Monoclonal Antibody (37.51) | Invitrogen | Cat#16-0281-82 |
| Purified anti-mouse IFN-β Antibody | BioLegend | Cat#519202 |
| Biotin anti-mouse IFN-β Antibody | BioLegend | Cat#508105 |
| Chemicals, and Recombinant Proteins | ||
| 2’3’-cGAMP | Invivogen | Cat#tlrl-nacga23–02 |
| 3’3’-cGAMP | Invivogen | Cat#tlrl-nacga |
| 2’3’-c-di-AM(PS)2 (Rp,Rp) | Invivogen | Cat#tlrl-nacda2r-01 |
| DMXAA | Invivogen | Cat#tlrl-dmx |
| 9-Oxo-10(9H)-acridineacetic acid (CMA) | Sigma | Cat#17927 |
| CellTrace™ CFSE Cell Proliferation Kit | Invivogen | Cat#C34554 |
| Annexin V Apoptosis Detection Kit | Biolegend | Cat#640914 |
| DNeasy Blood & Tissue Kits | QIAGEN | Cat#69506 |
| Protease Inhibitor Cocktail | Roche | Cat#11873580001 |
| Lipofectamine 2000 | ThermoFisher scientific | Cat#11668019 |
| TRI Reagent | Sigma | Cat#T9424 |
| Annexin V Binding Buffer | Biolegend | Cat#422201 |
| RIPA Lysis Buffer | Millipore | Cat#20–188 |
| ACK Lysing Buffer | ThermoFisher scientific | Cat#A1049201 |
| Pierce™ BCA Protein Assay Kit | ThermoFisher scientific | Cat#23225 |
| Polybrene | Sigma | Cat#TR-1003 |
| DNase I | Sigma | Cat#11284932001 |
| Collagenase | Sigma | Cat#C5138 |
| iScript™ Reverse Transcription Supermix | Bio-Rad | Cat#1708840 |
| iTaq™ Universal SYBR® Green Supermix | Bio-Rad | Cat#1725120 |
| Naive CD8a+ T Cell Isolation Kit | Miltenyi Biotec | Cat#130-096-543 |
| Pan T Cell Isolation Kit II | Miltenyi Biotec | Cat#130-095-130 |
| Dual-Luciferase® Reporter Assay System | Promega | Cat#E1960 |
| Recombinant Murine IL-2 | PEPROTECH | Cat#212–12 |
| C-176 STING inhibitor | BioVision | Cat#B2341 |
| Fixation/Permeabilization Solution Kit | BD | Cat#554714 |
| HRP Avidin | BioLegend | Cat#405103 |
| Recombinant Mouse IFN-β (ELISA Std.) | BioLegend | Cat#581309 |
| TMB Substate Set | BioLegend | Cat#421101 |
| Sulfuric acid (ELISA stop solution) | Millipore Sigma | Cat#160313 |
| Bacterial and Virus Strains | ||
| HSV-1 stain 11 | Dr. David Leib | N/A |
| VSV-GFP | Dr. Asit Pattnaik | N/A |
| Vaccinia virus (VACV) B5R-GFP | Dr. Bernard Moss | N/A |
| Recombinant DNA | ||
| Flag-cGAS PCMV | PMID:30886058 | N/A |
| Flag-STING PCMV | PMID:30886058 | N/A |
| Experimental Models: Cell Lines | ||
| HEK293T | ATCC | Cat# CRL-3216 |
| B16-F10 | Dr. Yang-Xin Fu | N/A |
| MC38 | Dr. Yang-Xin Fu | N/A |
| Experimental Models: Organisms/Strains | ||
| C56BL/6J | Jackson Laboratory | JAX: 000664 |
| Sting1S365A/S365A C56BL/6J | This study | N/A |
| Rag1−/− C56BL/6J | Jackson Laboratory | JAX:002216 |
| Sting1−/− C51BL/6J | Dr. Glen Barber | N/A |
| Irf3−/− C51BL/6J | Dr. Tada Taniguchi | N/A |
| Ifnar1−/− C51BL/6J | Jackson Laboratory | JAX:028288 |
| Software and Algorithms | ||
| Graphpad Prism | Graphpad | https://www.graphpad.com/ |
| FlowJo | FlowJo | https://www.flowjo.com |
| iDEP90 | iDEP | http://bioinformatics.sdstate.edu/idep/ |
| Ingenuity Pathway Analysis (IPA) | IPA | https://digitalinsights.qiagen.com/product-login/ |
| VisR | VisR | https://visrsoftware.github.io/ |
| ELISA Analysis | ELISA | https://elisaanalysis.com/ |
| Deposited data | ||
| RNA-seq data | BGI | NCBI GEO (accession number GSE149744) |