TABLE 1.

Comparison of morphological, physiological and genomic features of the novel Nitrolancea strains and Nl. hollandica.

Feature	Nl. hollandica strain Z*	Ca. Nl. copahuensis	Nl. hollandica
Morphology	Ovoid rods with tapered ends	Ovoid rods with tapered ends	Ovoid rods with tapered ends
Size [μm]	1.3–1.5 × 1.6–2.3	1.1 × 1.7	1–1.2 × 2–4
Temperature optimum	37°C	35–37°C	40°C
Temperature range for growth	19–45°C	19–45°C	20–46°C
Nitrite tolerance	150 mM	40 mM	75 mM
Nitrite optimum	3–10 mM	3–10 mM	5–20 mM
Km value (nitrite)	0.12–0.45 mM	0.22 mM	1 mM
μmax (nitrite) h–1	0.013	0.016	0.019
NxrA copies	2/4 (Z2/Z4)	2	4
Ammonium requirement	+	+	+
Ammonium optimum	5–20 mM	5–25 mM	5 mM
Ammonium tolerance	500 mM	500 mM	>200 mM
CO2 fixation	Calvin cycle	Calvin cycle	Calvin cycle
Rubisco	Type I	Type I	Type I
Isolation source	Centrate treatment reactor	Geothermal spring	Bioreactor

*Without differentiation between the genotypes Z2 and Z4.