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. 2020 Jul 10;8:540. doi: 10.3389/fcell.2020.00540

FIGURE 7.

FIGURE 7

Effects of lent-miR-138-5p on human NSCLC. After treatment with lent-miR-138-5p (Lent-M) or the corresponding negative control lentivirus (lent-N), PD-L1 expression on isolated human lung cancer cells was analyzed by qRT-PCR (A) or Western blot (B). Upon lent-miR-138-5p (lent-M) or lent-N treatment, the proliferation of human NSCLC cells was analyzed by flow cytometry detection of Ki67 (C) and the inhibition rate of the proliferation of human NSCLC cells was analyzed by MTT assay (D). (E) With lent-M, lent-N treatment, the inhibit ration of human DCs on isolated human lung cancer cells were analyzed. (F) Percentages of apoptotic tumor cells co-cultured with CD3+CD8+ human T cells (CTL) activated by human DCs pretreated or not with lent- miR138-5p or lent-N treated A549 cells. (G) The proliferation of human CD4+ T cocultured with human DCs pretreated or not with lent- miR138 or lent-N treated A549 cells (Three independent assays were performed; **P < 0.01, ***P < 0.001).