Figure 15.

Schematic overview of flow cytometric gating strategy for the identification of (A) Tc cells, (B) Treg cells, (C) Th1 cells, and (D) Th17 cells in liver, VAT, SAT, spleen, and blood. Lymphocyte populations were determined by FSC-A and SSC-A. Doublets and triplets were filtered out twice by plotting the area to height for both FSC and SSC. Concerning the fluorochrome-stained cells, positive populations were defined as being above the 99th percentile of FMO staining, indicated by the quadrants. -A, area; FSC, forward scatter; FMO, fluorescence minus one; -H; height; RORγt, RAR-related orphan receptor γt; SSC, side scatter.