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Representative images of liver histology showing the effect of HFHFD feeding. Original magnification: ×10. Compared with CD-fed mice, HFHFD-fed mice showed increasing degrees of hepatic steatosis (dark blue arrowhead), ballooning (yellow arrow), lobular inflammation (green triangle), and fibrosis (black star). Staining was performed with (A) H&E, (B) Oil-red-O, (C) CD45 immunohistochemistry, and (D) MT.
