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Effect of HFHFD feeding on VAT histology. Representative images of VAT stained with (A) H&E and (B) CD45 immunohistochemistry. Original magnification: ×10. Compared with CD-fed mice, HFHFD-fed mice showed increasing degrees of adipocyte hypertrophy (red arrowhead) and adipose tissue inflammation (blue triangle). (C) Adipocyte diameter. (D) Quantification of VAT inflammation through CD45 immunohistochemistry, expressed as the number of inflammatory foci per mm2.
