Figure 6.

Effect of diet reversal on phenotype. (A) Study design and legend. (B) Evolution total body weight. (C) Tissue weights. (D) Plasma cholesterol. (E) Plasma leptin. (F) GTT, absolute glycemia in mg/dL, and (G) ITT, percentage of glycemia at 0 minutes. The area under the curve was compared between the different groups. Medians with interquartile range are shown. (H) Plasma ALT. (I) NAS. N = 10–20 per group. (J) Quantification of hepatic steatosis through Oil-red-O staining, expressed as the percentage stained. (K) Morphometric quantification of macrovesicular steatosis on H&E-stained liver tissue, expressed as the percentage of macrovesicular steatosis. (L and M) Quantification of hepatic inflammation through (L) CD45 and (M) F4/80 immunohistochemistry, expressed as the number of inflammatory foci and crown-like structures per mm² respectively. (N) Fibrosis grade according to NASH-clinical research network. (O) Quantification of fibrosis through MT staining, expressed as the percentage stained. Medians are depicted with a horizontal black line. ^#P < .001, HFHFD-fed mice (including the DR group, before DR) vs CD-fed mice. ^$P < .01, 32 weeks HFHFD group vs the CD and DR groups. ∗∗P < .01, ∗∗∗P < .001 (Kruskal–Wallis, homogenous subsets are shown with a horizontal line above the data points).