Table 2.
Flow Cytometric Characterization of Tc, Treg, Th1, and Th17 Cells in Liver, VAT, SAT, Blood, and Spleen
| Liver |
VAT |
SAT |
Blood |
Spleen |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD | HFHFD | P | CD | HFHFD | P | CD | HFHFD | P | CD | HFHFD | P | CD | HFHFD | P | |
| Tc cells, % of XXX cells |
|||||||||||||||
| 10–15 wk | 41.4 | 46.6 | .021 | 27.4 | 25.5 | .569 | 37.1 | 39.4 | .120 | 37.2 | 34.7 | .217 | 34.1 | 32.9 | .061 |
| (36.8–44.9) | (38.0–52.2) | (24.0–30.1) | (19.3–28.3) | (27.6–40.0) | (33.9–45.6) | (34.8–39.6) | (33.7–39.9) | (32.6–38.2) | (29.7 - 35.8) | ||||||
| 20–25 wk | 46.9 | 42.5 | .336 | 26.3 | 41.2 | .002 | 46.6 | 41.1 | .172 | 40.8 | 37.2 | .006 | 33.0 | 34.1 | .509 |
| (36.5–52.9) | (40.2–46.6) | (24.5–35.4) | (33.3–44.1) | (45.3–48.0) | (37.2–44.4) | (38.8–44.2) | (35.9–40.4) | (31.1–39.1) | (29.7 - 36.5) | ||||||
| 32 wk | 49.2 | 49.5 | .809 | 18.7 | 40.1 | <.001 | 34.8 | 38.8 | .442 | 41.0 | 38.5 | .049 | 33.6 | 29.7 | .043 |
| (44.5–54.6) | (47.6–53.9) | (12.7–22.6) | (38.5–43.7) | (28.3–44.9) | (35.1–49.5) | (38.9–43.7) | (34.9–40.2) | (28.9–38.3) | (24.5 - 33.8) | ||||||
| Treg cells, % of XXX cells |
|||||||||||||||
| 10–15 wk | 1.6 | 1.6 | .899 | 16.6 | 18.9 | .270 | 8.9 | 5.7 | 1.000 | 5.3 | 4.3 | .611 | 5.7 | 6.0 | .830 |
| (0.8–2.3) | (1.1–2.9) | (13.2–20.5) | (6.4–36.4) | (5.0–10.9) | (4.6–11.3) | (3.0–6.7) | (3.5–6.5) | (3.8–13.7) | (4.3 - 8.2) | ||||||
| 20–25 wk | 2.3 | 2.2 | .790 | 19.9 | 12.7 | .022 | 3.1 | 7.9 | .432 | 6.3 | 7.3 | .083 | 3.1 | 3.6 | .478 |
| (1.8–3.2) | (1.7–3.5) | (13.7–36.2) | (9.7–19.1) | (2.8–3.4) | (4.1–10.0) | (0.9–7.2) | (4.0–8.2) | (2.3–5.7) | (2.7 - 5.4) | ||||||
| 32 wk | 1.9 | 4.4 | .055 | 30.9 | 7.8 | <.001 | 6.9 | 6.3 | .791 | 6.9 | 7.8 | .512 | 2.1 | 5.0 | .604 |
| (1.0–3.0) | (2.7–7.7) | (15.4–54.7) | (4.7–10.6) | (5.1–12.2) | (5.1–9.0) | (4.0–7.7) | (5.7–8.5) | (1.0–7.1) | (0.9 - 13.1) | ||||||
| Th1 cells, % of XXX cells |
|||||||||||||||
| 10–15 wk | 1.1 | 1.8 | .280 | 0.9 | 0.9 | .711 | 2.5 | 1.2 | .238 | 1.9 | 1.5 | .376 | 2.2 | 1.9 | .941 |
| (0.8–1.5) | (0.6–3.8) | (0.9–5.4) | (0.7–3.3) | (1.6–7.6) | (0.9–7.3) | (1.8–2.4) | (0.7–2.8) | (1.5–2.8) | (1.7 - 3.5) | ||||||
| 20–25 wk | 1.0 | 0.7 | .852 | 0.8 | 0.6 | .494 | 2.2 | 1.1 | .615 | 3.9 | 2.9 | .215 | 2.8 | 3.7 | .714 |
| (0.4–2.9) | (0.5–1.5) | (0.1–1.3) | (0.3–0.9) | (0.6–3.8) | (0.8–1.7) | (2.9–5.0) | (2.1–4.7) | (1.6–5.2) | (1.8 - 4.0) | ||||||
| 32 wk | 0.6 | 0.7 | .809 | 0.8 | 0.5 | .151 | 3.2 | 1.4 | .375 | 2.5 | 1.6 | .512 | 2.3 | 2.2 | 1.000 |
| (0.4–2.5) | (0.4–1.2) | (0.6–2.2) | (0.3–0.9) | (1.7–6.3) | (1.0–3.4) | (0.6–3.1) | (0.8–5.1) | (0.9–4.3) | (1.4 - 5.9) | ||||||
| Th17 cells, % of XXX cells |
|||||||||||||||
| 10–15 wk | 1.2 | 1.4 | .202 | 1.1 | 1.1 | .711 | 1.7 | 2.6 | .272 | 1.3 | 1.2 | .538 | 2.0 | 0.6 | .370 |
| (0.9–1.6) | (1.3–2.8) | (0.8–1.7) | (0.8–2.0) | (0.6–5.6) | (1.4–5.8) | (0.6–3.3) | (0.9–2.2) | (0.3–5.4) | (0.4 - 2.7) | ||||||
| 20–25 wk | 2.5 | 2.9 | .018 | 0.3 | 1.4 | .001 | 0.3 | 1.8 | .198 | 1.3 | 1.3 | .837 | 0.8 | 0.9 | .914 |
| (2.0–2.9) | (2.5–4.4) | (0.0–1.0) | (0.9–2.9) | (0.0–0.6) | (1.1–3.4) | (0.6–2.6) | (0.7–3.2) | (0.5–7.2) | (0.4 - 2.0) | ||||||
| 32 wk | 1.5 | 3.0 | <.001 | 1.1 | 3.9 | <.001 | 0.9 | 2.5 | .085 | 2.0 | 3.3 | .010 | 1.1 | 2.4 | 1.000 |
| (1.1–2.2) | (2.2–7.6) | (0.8–1.3) | (2.2–6.5) | (0.2–1.2) | (1.0–4.0) | (1.0–3.0) | (2.8–3.5) | (0.5–4.7) | (1.0 - 3.0) | ||||||
NOTE. Data are presented as medians (interquartile range). Statistical analysis was performed with a Mann–Whitney U test. Significant P values are in bold.