Table 1.

Primer sequences, annealing temperatures, cycle numbers, and product sizes for semiquantitative RT-PCR.

Gene (abbreviation)	Primer sequence forward/reverse	Annealing temperature (°C)	Semiquantitative RT-PCR cycles	Size of amplified products (bp)
COL-1	5′-CCCGGGTTTCAGAGACAACTTC-3′/5′-TCCACATGCTTTATTCCAGCAATC-3′	61	23	148
PLAP-1	5′-ATGGGAGTCTTGCTAACATACCAC-3′/5′-CAGAAGTCATTTACTCCCACTCTTG-3′	60	30	154
POSTN	5′-CACAACCTGGAGACTGGACA-3′/5′-TGCTTCCTTGTGTGGTCTTT-3′	59	25	198
GAPDH	5′-ACCACAGTCCATGCCATCCAC-3′/5′-TCCACCACCCTGTTGCTGTA-3′	60	20	452