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. 2020 Jul 8;2020:9672673. doi: 10.1155/2020/9672673

Table 2.

Primer sequences, annealing temperatures, cycle numbers, and product sizes for quantitative RT-PCR.

Gene (abbreviation) Primer sequence forward/reverse Annealing temperature (°C) Quantitative RT-PCR cycles Size of amplified products (bp)
p75NTR 5′-CCGAGGCACCACCGACAACC-3′/5′-TGCTTGCAGCTGTTCCACCTCT-3′ 60 40 108
N-cadherin 5′-GAAAGACCCATCCACGCCGAGC-3′/5′-TCAGCCGCTTTAAGGCCCTCATT-3′ 60 40 101
NANOG 5′-TCCAACATCCTGAACCTCAGCTA-3′/5′-AGGTTCCCAGTCGGGTTCAC-3′ 60 40 193
MEST 5′-ATGGGCCATTGGATCCTGTA-3′/5′-CAGAAGGAGTTGATGAAGCCCA-3′ 60 40 169
CCND1 5′-AGGAGAACAAACAGATCA-3′/5′-TAGGACAGGAAGTTGTTG-3′ 60 40 163
CCNE1 5′-GCCAGCCTTGGGACAATAATG-3′/5′-CTTGCACGTTGAGTTTGGGT-3′ 60 40 104
ALP 5′-GGACCATTCCCACGTCTTCAC-3′/5′-CCTTGTAGCCAGGCCCATTG-3′ 60 40 137
Osterix 5′-GCCATTCTGGGCTTGGGTATC-3′/5′-GAAGCCGGAGTGCAGGTATCA-3′ 60 40 129
PPARγ 5′-TATTCTCAGTGGAGACCGCC-3′/5′-TGAGGACTCAGGGTGGTTCA-3′ 60 40 115
C/EBPα 5′-GGTGGACAAGAACAGCAAGGA-3′/5′-GTCATTGTCACTGGTCAGCTC-3′ 60 40 135
COL-2 5′-CAACCAGGACCAAAGGGACA-3′/5′-ACCTTTGTCACCACGATCCC-3′ 60 40 129
Aggrecan 5′-TGGTGATGATCTGGCACGAG-3′/5′-CGTGAGCTCCGCTTCTGTAG-3′ 60 40 124
COL-1 5′-CCCGGGTTTCAGAGACAACTTC-3′/5′-TCCACATGCTTTATTCCAGCAATC-3′ 60 40 148
PLAP-1 5′-ATGGGAGTCTTGCTAACATACCAC-3′/5′-CAGAAGTCATTTACTCCCACTCTTG-3′ 60 40 154
POSTN 5′-CACAACCTGGAGACTGGACA-3′/5′-TGCTTCCTTGTGTGGTCTTT-3′ 60 40 198
β-Actin 5′-ATTGCCGACAGGATGCAGA-3′/5′-GAGTACTTGCGCTCAGGAGGA-3′ 60 40 89