Fig. 1.

Kinetic analysis of synaptosomal [³H]DA uptake was determined in the PFC of iTat-tg mice after 7 or 14 days’ administration of saline or Dox. Synaptosomes were incubated with a range of mixed DA concentrations (0.1–5 µM, final concentration) containing a fixed concentration (12 nM) of [³H]DA. The V_max and K_m values for [³H]DA uptake via DAT (A and C) or NET (B and D) in the PFC of iTat-tg (iTat) mice after 7 (A and B) or 14 (C and D) days’ administration of saline or Dox were calculated using nonlinear regression analysis with a one-site binding parameter and represent the means from five to seven independent experiments ± S.E.M. *P < 0.05; **P < 0.01 compared with saline control group. SAL, saline.