TABLE 1.
Kinetic properties of [3H]DA uptake and [3H]WIN 35,428 binding or [3H]nisoxetine binding in iTat-tg mice
Data are expressed as means ± S.E.M. values from five to seven independent experiments performed in duplicate.
| Striatum (DAT) | Hippocampus (NET) | |||||
|---|---|---|---|---|---|---|
| Vmax (pmol/min per milligram) | Km (nM) | Vmax (pmol/min per milligram) | Km (nM) | |||
| Treatment duration | 7 days | iTat-tg saline | 15.6 ± 1.4 | 97.3 ± 6.6 | 0.898 ± 0.12 | 102 ± 23 |
| iTat-tg Dox | 15.6 ± 1.6 | 91.1 ± 3.4 | 0.926 ± 0.077 | 75.4 ± 14 | ||
| 14 days | iTat-tg saline | 67.6 ± 8.5 | 140 ± 11 | 1.96 ± 0.19 | 122 ± 18 | |
| iTat-tg Dox | 68.4 ± 8.9 | 141 ± 10 | 1.86 ± 0.26 | 115 ± 24 | ||
| Bmax pmol/mg | Kd (nM) | Bmax pmol/mg | Kd (nM) | |||
| 7 days | iTat-tg saline | 22.9 ± 3.7 | 20.34 ± 5.3 | 0.611 ± 0.10 | 13.4 ± 3.3 | |
| iTat-tg Dox | 25.4 ± 5.4 | 18.3 ± 1.8 | 0.552 ± 0.096 | 15.4 ± 2.0 | ||