Table 1.
Expression level of the R27 genes with altered expression at either 25 or 37°C in cultures grown up to mid-logarithmic phase.
| Gene | 25°C | 37°C | FC | Gene name and predicted function | ||
|---|---|---|---|---|---|---|
| R0007 | 1172 | 309 | 3.79 | trhW (AN), pilus assembly | ||
| R0008 | 299 | 101 | 2.97 | trhP (AN), peptidase | ||
| R0025 | 322 | 128 | 2.52 | trhV (AC), lipoprotein | ||
| R0027 | 528 | 176 | 2.99 | htdT (AC) | ||
| R0029 | 664 | 185 | 3.59 | trhB (AC), Mpf formation | ||
| R0030 | 558 | 138 | 4.04 | htdO (AC) | ||
| tra genes | FC>+2 | R0031 | 465 | 102 | 4.56 | trhK (AC), pilus assembly |
| R0032 | 1337 | 201 | 6.64 | trhE (AC), pilus assembly | ||
| R0033 | 1424 | 70 | 20.44 | trhL (AC), pilus assembly | ||
| R0034 | 1536 | 107 | 14.35 | trhA (AC), pilus major subunit | ||
| R0121 | 1207 | 145 | 8.32 | (H) | ||
| R0122 | 511 | 41 | 12.45 | traH (H), relaxosome protein | ||
| R0123 | 1669 | 510 | 3.27 | trhR (R), regulator | ||
| R0126 | 129 | 20 | 6.47 | trhF (F), pilus assembly | ||
| FC < -2 | R0005 | 190 | 393 | −2.06 | trhN (AN), Mpf complex stability | |
| R0001 | 1306 | 147 | 8.91 | |||
| R0002 | 172 | 19 | 9.18 | |||
| R0042 | 6132 | 2278 | 2.69 | partition protein | ||
| R0129 | 169 | 45 | 3.76 | |||
| R0130 | 183 | 59 | 3.13 | bfpH, trbN-like protein | ||
| R0135 | 1433 | 425 | 3.37 | dsbA, outer membrane protein | ||
| FC>+2 | R0153 | 692 | 242 | 2.86 | partition protein | |
| R0154 | 1714 | 362 | 4.73 | cythosine methylase | ||
| R0200 | 129 | 36 | 3.58 | |||
| R0204 | 403 | 77 | 5.25 | |||
| R0205 | 352 | 40 | 8.72 | |||
| R0206 | 197 | 26 | 7.74 | |||
| R0207 | 921 | 86 | 10.71 | |||
| R0208 | 268 | 13 | 20.23 | |||
| R0209 | 310 | 18 | 17.00 | |||
| R0210 | 1201 | 52 | 22.90 | |||
| R0023 | 62 | 181 | −2.92 | |||
| R0036 | 585 | 1422 | −2.43 | repHIA, replication protein | ||
| R0044 | 42 | 124 | −2.95 | |||
| R0048 | 562 | 1190 | −2.12 | |||
| R0052 | 154 | 359 | −2.33 | |||
| R0053 | 115 | 282 | −2.46 | |||
| non- | R0058 | 176 | 395 | −2.25 | ||
| tra genes | R0067 | 98 | 218 | −2.23 | ||
| R0069 | 47 | 118 | −2.54 | |||
| R0070 | 558 | 1671 | −3.00 | |||
| R0075 | 67 | 177 | −2.65 | |||
| R0083 | 231 | 914 | −3.95 | tetC, transcriptional regulator | ||
| R0094 | 1306 | 3893 | −2.98 | insB1, IS1 transposase | ||
| FC < -2 | R0095 | 6229 | 12567 | −2.02 | insA, IS1 transposase | |
| R0097 | 191 | 397 | −2.08 | yigE | ||
| R0139 | 233 | 475 | −2.03 | |||
| R0140 | 119 | 378 | −3.18 | |||
| R0142 | 1126 | 5777 | −5.13 | |||
| R0143 | 372 | 779 | −2.09 | |||
| R0148 | 428 | 993 | −2.32 | Transposase | ||
| R0149 | 58 | 224 | −3.89 | |||
| R0151 | 189 | 401 | −2.12 | |||
| R0159 | 725 | 1932 | −2.66 | |||
| R0160 | 122 | 325 | −2.66 | |||
| R0162 | 298 | 637 | −2.13 | |||
| R0166 | 259 | 867 | −3.34 | |||
| R0175 | 399 | 893 | −2.24 | |||
| R0176 | 154 | 807 | −5.24 | |||
| R0177 | 2327 | 6441 | −2.77 | insB, transposase | ||
| R0180 | 3294 | 9075 | −2.76 | insB, transposase | ||
| R0182 | 117 | 289 | −2.47 | hha, regulator | ||
| R0183 | 1567 | 3694 | −2.36 | |||
| R0195 | 1684 | 5041 | −2.99 | insD, transposase |
The fold change of the fluorescence arbitrary units and the predicted function is indicated. The genes are divided in tra and non-tra genes. Attending to the fold change (FC), genes are classified in derepressed at 25°C (FC>+2) and repressed at 25°C (FC < -2).