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. Author manuscript; available in PMC: 2020 Oct 1.

Published in final edited form as: Toxicol Lett. 2020 May 24;331:82–91. doi: 10.1016/j.toxlet.2020.05.023

Fig. 2 — Protein levels of HIF-1α and its downstream targets in PC12 and BRL cells after treatment with methylmercury (MeHg). A) Western blotting of HIF-1α in cells treated with different concentrations (1–10 μM) of MeHg for 0.5 h. B) Quantitative RT-PCR was used to measure HIF-1α mRNA expression. C) Western blotting was used to detect the protein level of HIF-1β. D) The downstream proteins of HIF-1α glucose transporter-1 (GLUT-1), vascular endothelial growth factor-A (VEGF-A), and erythropoietin (EPO) after exposure to various concentrations (1–10 μM) of MeHg. β-Actin served as a loading control. Data show mean ± standard deviation (n = 3).

*p < 0.05, compared with the control group. Control groups were treated with free media without any agents. Statistical analysis was performed by one-way analysis of variance followed by a Dunnett test, a multiple comparison procedure.

Fig. 2 — Protein levels of HIF-1α and its downstream targets in PC12 and BRL cells after treatment with methylmercury (MeHg). A) Western blotting of HIF-1α in cells treated with different concentrations (1–10 μM) of MeHg for 0.5 h. B) Quantitative RT-PCR was used to measure HIF-1α mRNA expression. C) Western blotting was used to detect the protein level of HIF-1β. D) The downstream proteins of HIF-1α glucose transporter-1 (GLUT-1), vascular endothelial growth factor-A (VEGF-A), and erythropoietin (EPO) after exposure to various concentrations (1–10 μM) of MeHg. β-Actin served as a loading control. Data show mean ± standard deviation (n = 3).

*p < 0.05, compared with the control group. Control groups were treated with free media without any agents. Statistical analysis was performed by one-way analysis of variance followed by a Dunnett test, a multiple comparison procedure.