Table 1. Percentage (%) Recovery of Lectin at Different Purification Stepsa.
| procedure/step | fraction volume (mL) | protein (mg/mL) | total protein (mg) | recovery (%) | hemagglutination unit (HU) | total hemagglutination activity | specific hemagglutination activity | fold purification |
|---|---|---|---|---|---|---|---|---|
| crude | 23 | 25.62 | 589.26 | 100.00 | 1 | 23 | 0.039 | 1 |
| (NH4)2SO4 precipitation | 13 | 15.76 | 204.88 | 034.76 | 2 | 26 | 0.126 | 3.230 |
| lactose affinity column | 15 | 08.52 | 127.80 | 021.68 | 7 | 105 | 0.466 | 11.94 |
a
Total hemagglutination activity = HU × volume (mL)/specific hemagglutination activity = HU/protein (mg/mL).