Figure 4.

Kinetic analysis of the two-step translocation process. (a) Voltage dependence of the logarithm of rate constant k₀₁. (b) Voltage dependence of the logarithm of rate constants k₁₂ and k₁₂′. (c) Voltage dependence of the logarithm of rate constant k₂₁′. Each data point plotted in panels a (first association) and b (initial binding) was obtained from the mean value of at least 10 independent measurements. Each data point plotted in panels b (rebinding) and c (unbinding) was generated from thousands of reversible binding events. The derived rate constants are provided in Table S1.