Figure 2.

UCHL1 activity profiling using ABP 2 in HEK293 cells. (a) Chemical proteomics workflow. Cells were incubated with 2 and labeled proteins ligated via CuAAC to AzTB for in-gel fluorescence and/or affinity enrichment for immunoblotting and proteomic profiling. (b) In-gel fluorescence shows dose-dependent labeling by 2. (c) Probe-labeled protein identification by enrichment and immunoblotting.