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UCHL1 labeling with 1 or 2 exclusively at catalytic Cys90 in vitro and in intact cells. (a) LC-ESI demonstrating single UCHL1 covalent modification with 1 or 2. (b) HeLa cells lacking endogenous UCHL1, transfected to express UCHL1 wild-type (WT) or catalytic cysteine mutants (C90A and C90S); pulldown following treatment with 2 and AzTB functionalization confirms specific Cys90 labeling.
