Skip to main content
. Author manuscript; available in PMC: 2021 May 7.
Published in final edited form as: Cell Stem Cell. 2020 Mar 5;26(5):766–781.e9. doi: 10.1016/j.stem.2020.02.002

Figure 3. Layer Expansion in SNOs over Long-Term Cultures.

Figure 3.

(A) Sample tiling confocal images of cortical structures in SNOs (top panel) and unsliced organoids (bottom panel), for immunostaining of CTIP2, SOX2, and TBR2. Dashed lines mark the pial surfaces and the boundaries of the VZ, oSVZ, and CP. The laminar structures in days 120 and 150 unsliced organoids are disorganized and thus not marked by dashed lines. Scale bar, 100 μm.

(B and C) Quantifications of the total thickness (B) and CP thickness (C). Values represent mean ± SD (n ≥ 10 and 5 organoids for SNOs and unsliced organoids for each iPSC line, respectively; *p < 0.05; **p < 0.005; ***p < 0.0005; Student’s t test).