Figure 2.

Proteomics, transcriptomics and FUS interactomics analyses in motoneurons. (a) Table showing genes encoding for differentially expressed proteins, ordered by fold change, and differentially expressed mRNAs, in FUS^P525L motoneurons compared to FUS^WT ones. Color code: blue, downregulated; red, upregulated. (b) Correlation between transcript and protein levels of genes enlisted in (a). PCC: Pearson correlation coefficient; p: p value. (c) Table showing miR-375 target genes that encode for differentially expressed proteins in FUS^P525L motoneurons. Color code: blue, downregulated; red, upregulated. (d) Venn diagram showing the overlap between proteins that are altered, in any direction, in FUS^P525L motoneurons (“MASS-SPEC”) and transcripts that are bound in intronic regions by endogenous FUS^WT (“INTRON ENDO”) or exogenous FLAG-FUS^WT (“INTRON FLAG”). (e) Venn diagram showing the overlap between proteins that are altered, in any direction, in FUS^P525L motoneurons (“MASS-SPEC”) and transcripts that are bound in the 3′UTR by endogenous FUS^P525L (“3′UTR ENDO”) or exogenous FLAG-FUS^P525L (“3′UTR FLAG”). In (d, e), a red box indicates that the Fisher’s exact test p-values are considered significant. Specifically, p < 0.01 for the overlap between INTRON FLAG and MASS-SPEC in (d) and for the overlap between 3′UTR ENDO and MASS-SPEC in (e). Complete list of p-values are reported in Supplementary Fig. S2 online. The drawings have been taken by Maria Giovanna Garone.