Figure 2.

Biodistribution of DIR-labeled BMM in healthy mice by IVIS. DIR-labeled BMM were administered in healthy mice (12 mo. of age) (A, D) i.v. (4 × 10⁶ cells/200 µL), (B, E) i.p. (4 × 10⁶ cells/200 µL), or (C, F) i.t. (1 × 10⁶ cells/50 µL). Images were recorded (A–C) in live animals at different time points, and (D–F) in main organs, liver (1), spleen (2), kidney (3), lungs (4), and brain (5), collected at the endpoint (72 h). Prone representative images of animals i.v., and i.p. suggest that DIR-BMM accumulate in the brain, although to a much lesser extent than same treatments in Parkin Q311(X)A mice (Fig. 1). Little if any DIR signal was observed in live animals after i.t. administration in healthy animals. Accumulation of labeled macrophages was also observed in the main peripheral organs.