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. 2020 Jul 16;11:3568. doi: 10.1038/s41467-020-17341-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative images of posterior midguts. Transgenes were induced for 2 days with esg^ts. Scale bar, 50 µm. b Quantification of disseminated cells residing on the surface of VM. N = 20 (esg^ts), N = 15 (esg^ts > piezo-i⁸⁴⁸⁸), N = 14 (esg^ts > piezo-i^v2796), N = 15 (esg^ts > Ras^V12), N = 9 (esg^ts > Ras^V12, piezo-i⁸⁴⁸⁸), N = 8 (esg^ts > Ras^V12, piezo-i^v2796) biological replicates. c Quantification of disseminated cells detected on the surface of VM. 100 µM GdCl₃-supplemented food was fed for 2 days while expressing Ras^V12 with esg^ts. N = 11 (esg^ts > Ras^V12), N = 17 (esg^ts > Ras^V12) biological replicates. d Quantification of discontinuous longitudinal muscles. N = 21 (esg^ts), N = 18 (esg^ts > piezo-i⁸⁴⁸⁸), N = 18 (esg^ts > piezo-i^v2796), N = 15 (esg^ts > Ras^V12), N = 14 (esg^ts > Ras^V12, piezo-i⁸⁴⁸⁸), N = 18 (esg^ts > Ras^V12, piezo-i^v2796) biological replicates. e Quantification of vesicles. N = 10 biological replicates for each genotype. In (b–e), mean ± SEMs are shown with individual data points. Data were analyzed by two-tailed unpaired Student’s t-test, asterisks indicate statistical significance (*P < 0.01), and P values are indicated in graph.