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. 2020 Jul 16;10:11802. doi: 10.1038/s41598-020-67877-8

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth inhibition activity of AMA1-RON2L, RIPR, CyRPA and RH5 antibodies. Increasing concentrations of purified total IgG from rabbits immunized with RH5 (a), AMA1-RON2L (b), RIPR (c), CyRPA (d) or Control IgG (e) were tested for growth inhibition of 3D7 clone parasites. Data shown are the mean and SEM of at least two independent experiments each with three replicate wells. Dashed line shows 50% inhibition. For RH5 (a), GIA was done with a pool of total IgG from rabbits immunized with RH5. For the other antigens or negative control (b–e), two total IgG samples from two rabbits were tested individually (two lines per antigen).