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. 2020 Jul 16;10:11738. doi: 10.1038/s41598-020-68462-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the complete sequence of the Shiga toxin (Stx) 2a phage of Stx-enteroaggregative E. coli O86:H27, O104:H4, hybrid Shigatoxigenic and enterotoxigenic E. coli O2:H27, and O111:H2. Arrows represent the CDSs, and major genes are coloured as shown in the legend. BLASTn and visualization were performed with GenomeMatcher software. The number of single nucleotide polymorphisms (SNPs) and insertions and deletions (indels) to the phage of O104:H4 are shown on the right. Red and blue asterisks show the position of SNPs and deletion, respectively, compared to the phage of O104:H4.