Fig. 2. Mesoscale calcium responses to coherent motion in RDKs.

a Schematic of the RDK stimulus. A single repeat of the stimulus contains dots drifting at varying coherence values across the trial duration (bottom). Over multiple repeats, the coherence values remain constant while the dot positions and directions are randomized every trial. b Top: map from a single experiment showing coherent motion correlation to RDKs across posterior cortex. Bottom: single pixel response (red) over the course of a single presentation of the stimulus overlaid on the coherence value trace; pixel-wise coherent motion correlation is calculated as the Pearson correlation between these two signals (r = 0.35). c Mean coherent motion correlation map across all imaged mice (n = 10 sessions over 10 mice). Individual maps are transformed onto a common coordinate system for comparison across mice. d Pixel-wise density plot of RDK vs. natural movie motion correlation across all mice to show similarity of motion correlation across visual stimuli (r = 0.71). Density plots are similar to scatter plots but provide additional information about the density (but not strength) at each coordinate, with warmer colors indicating a higher density of observation pairs (see Methods). e Natural movie versus RDK motion correlation for each mouse (r = 0.30 ± 0.02, n = 10, p = 1.0 × 10⁻⁷; two-tailed single-sample t test).